FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45981735
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC70250.017.9997484
TTCGGCG74850.017.58538244
AACGCTA103300.016.81431823
ACGCTAC106350.016.45939324
CAGACCG78600.016.4024433
TCGGCGC81500.016.15002845
CGTTACG12150.015.73952921
CGCTACC110950.015.716225
GTCCGTT35200.015.6672111
CGCAAAC48550.015.38586825
AGACGCT215700.014.94960341
TTAGTTA56200.014.771488
CCCCGCA209050.014.70473835
AAAACGC120850.014.53963321
CCGCGTT87100.014.4645137
AAGCGGC213350.014.44851728
CCCCCGC223150.014.32958534
CGTACGC91300.014.2933385
GAAAACG123550.014.27627220
GCCGGCT131300.014.2463811
Produced by FastQC (version 0.11.5)