FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45981735
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG129650.011.2302838
AGCTTAG132250.011.09600245
GGCATAG133850.010.89425535
GGGAGGT205050.010.455543
ACTCTCC205200.09.98794411
GGTCACT208400.09.8552047
TGGCATA151350.09.76836734
CATAGGG151150.09.6178637
GTCACTC225250.09.2977618
GGAGGTC233500.09.29717354
TCAGACG79000.09.2548552
GCATAGG158250.09.243299536
GAGGTCA237600.09.1934715
GAGCTTA165400.08.9128644
TCACTCT243850.08.7270239
CGTGGCG111900.08.6850291
CACTCTC239550.08.62073510
ACACTCG91100.08.5688314
CGACCCG113300.08.4977336
CACTCGA85500.08.4195825
Produced by FastQC (version 0.11.5)