FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44736162
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT870550.19459648773625238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC216550.019.2631424
GTCCGTT40100.018.1918681
CAGACCG231700.018.1201253
GACCGCG231750.017.951175
ACCGCGT237900.017.5324976
GAACGAT242750.017.2948345
CCGCGTT250650.016.6496147
CGCGTTC254850.016.392868
AGAACGA269850.015.60955544
TCAGACC279000.015.3223342
AATACGG289350.014.46551732
GCGTTCT293350.014.3180649
TACGGAG293950.014.2701234
ATACGGA296550.014.23606933
CTCAGAC326800.014.1788171
CCCGTAT27700.013.9805851
CAATACG303400.013.94268731
AAGAACG301800.013.94229743
CCGTACG85850.013.9164924
CGTTACG12950.013.89886321
Produced by FastQC (version 0.11.5)