FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50139315
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG171650.013.56712138
GTTGTCG29500.013.50208926
AGCTTAG172850.013.39785545
GGCATAG173700.013.09599835
GGGAGGT259150.012.6084413
GGAGGTC267550.012.0713024
TGGCATA198750.011.46887434
GAGCTTA201750.011.37884844
GTCACTC278400.011.1034048
ACTCTCC281550.011.01293511
CATAGGG213750.010.82112737
TGTCGTT39000.010.50227528
GCATAGG227350.010.18374836
GAGGTCA323750.010.0816625
TCACTCT323700.010.0152279
GGTCACT311450.09.9751667
CACTCTC312950.09.9279110
ATATAGC58250.09.8862386
TGGGAGG261150.09.5805692
TAGGGGA328900.09.28261339
Produced by FastQC (version 0.11.5)