FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46958794
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT670390.1427613324141161No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG628160.13376834166567397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT172250.018.61440545
AACGCTA196150.018.40986623
AGACCGC173750.018.3874474
ACGCTAC196300.018.21264824
TCACGCT205200.018.1341638
CCGGCTT223700.018.0933482
CGCTACC199500.017.87534525
TTCACGC213350.017.4730727
CAGACCG184600.017.4162713
GCCGGCT223100.017.3901021
AAAACGC213150.016.98290421
GAAAACG216800.016.70732520
ACGCTCA232250.016.6419110
ACCGCGT193600.016.5364116
AAACGCT223900.016.26830722
GTCCGTT28300.016.151991
GACCGCG200250.015.9766235
CCGCGTT202200.015.8218727
CGGCTTC261250.015.5702933
CGCGTTC206200.015.5149498
Produced by FastQC (version 0.11.5)