FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51895881
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC539720.10400054678713327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA104600.016.9504515
CCTACTC106200.016.41841718
GACCGCG68350.015.5040985
ACCGCGT68350.015.4379226
AGACGCT252300.014.67944941
AGACCGC73350.014.4779134
GTCCGTT40400.014.2613961
CCCCGCA241800.014.24660835
GCAGACG261950.014.1389339
CAGACCG76800.014.0912013
CCGCAGA266700.013.98878837
CGAACGA38350.013.90514632
CCCCCGC257450.013.86126534
GCCCCCG258100.013.84379333
ACGAACG39250.013.8153831
AAGCGGC241100.013.792541528
CGTTACG18250.013.6841421
CCCGTAT37300.013.6365061
CCCCCCT42750.013.5827311
CAGACGC274200.013.57291740
Produced by FastQC (version 0.11.5)