FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51895881
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG124650.012.64045538
AGCTTAG127900.012.28397545
GGCATAG126500.012.11759735
GGGAGGT200800.011.3698783
CATAGGG148650.010.44860637
GGAGGTC223200.010.1986514
GCATAGG153450.010.13610436
GAGCTTA156650.010.1156344
TGGCATA155600.010.08283434
GGTCACT224050.09.6780837
ACTCTCC241250.09.27711711
GTTGTCG20950.09.23583826
TAGGGGA232550.09.1952339
CGCGTTC19550.08.74419714
GCGTTCG18300.08.72685615
GAGGTCA276100.08.7008425
GTCACTC257000.08.6999178
CGTAGGG72400.08.39458737
CGTGGCG69450.08.3248871
CGACCCG71350.08.1335796
Produced by FastQC (version 0.11.5)