FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50852381
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1626000.3197490398728822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT290750.026.13291545
AGACCGC352150.021.8935384
AGAACGA356650.021.34236344
CAGACCG364500.021.2629323
ACCGCGT363250.021.2546186
CCGCGTT372350.020.719667
GACCGCG371900.020.7187545
CGCGTTC376100.020.5186448
TCAGACC409250.019.1689932
GCGTTCT411200.018.8492249
AAGAACG405250.018.79442243
AATACGG415950.018.30479632
ATACGGA418800.018.19102733
TACGGAG419650.018.08984434
CTCAGAC465200.017.8709681
CAATACG435450.017.55225431
ACGGAGA439750.017.29369435
CGGAGAG447100.017.009436
CCCAATA455900.016.85700429
GTTCCTA96800.016.57772315
Produced by FastQC (version 0.11.5)