FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48142651
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA542560.1126984054118665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT285100.012.2936833
AGCTTAG183500.012.26478745
GGAGGTC284300.012.0354374
ATAGGGG195750.011.53021538
GGCATAG196600.011.273725535
GACGCAA76400.010.722744
GTCACTC316150.010.5952018
GGTCACT316800.010.5944247
ACTCTCC323400.010.477097511
GAGCTTA211900.010.47230144
GTTGTCG20600.010.375710526
ACGCAAG82150.010.13655245
TGGCATA224850.09.90751534
CACCCGG87850.09.478761
CATAGGG248600.09.1061137
TGGGAGG270900.08.9105082
GCATAGG260050.08.75703236
GAGGTCA411300.08.6528125
AGGTCAC411100.08.5527656
TAGGGGA389300.08.53183639
Produced by FastQC (version 0.11.5)