FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47382929
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG566040.11946074502908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG38300.018.68057621
GCCGGCT171550.018.2839411
CCGGCTT217300.017.5210192
GACCGCG118900.016.5026045
TTCACGC206750.016.2033967
AGACCGC122350.016.1476144
TTCGACG24750.016.0899586
ACCGCGT125100.015.7904356
TCACGCT212300.015.7268148
CAGACCG130850.015.3394213
CCCGTAT41300.014.93860151
GTCCGTT43500.014.7007161
GTTACGA49000.014.647264522
ACGCGTC16300.014.49418540
CGGCTTC266300.014.4322663
ACGCTCA237150.014.23070310
CCGCGTT138950.014.2165047
CCCCCCT36800.014.073091
CTCCGAT49400.013.9933221
CCCCGCT57950.013.8326691
Produced by FastQC (version 0.11.5)