FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47382929
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG760010.16039742920915676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG35850.016.25635126
GGGAGGT377750.014.3882953
GGAGGTC381350.014.0244484
ATATAGC55800.013.5839616
GAGGTCA414750.013.0256235
AGGTCAC434100.012.4403666
GGTCACT431750.012.2272287
AGCTTAG326250.012.10156945
TGTCGTT49700.012.0433428
GTCACTC442450.011.8148668
ATAGGGG337750.011.67425838
TGGGAGG356600.011.6467262
CTGGGAG397000.011.3984971
ACTCTCC468300.011.22191811
GGCATAG347250.011.20591735
TCACTCT476250.011.0857089
CACCCGG93850.011.023861
CACTCTC470600.010.99946610
GAGCTTA359200.010.95387444
ACCCGGG92850.010.8496182
Produced by FastQC (version 0.11.5)