FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47334791
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG597140.12615245306565312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC107050.015.972475
AGCTTAG186800.015.36376745
GGCATAG195600.014.71065835
GGTCACT297900.014.50896557
GTCACTC309950.014.1068828
AGGTCAC334250.013.4493386
TGGCATA218150.013.34470734
GAGCTTA217950.013.22052644
ACACGGA125700.013.17672916
GAGGTCA344650.013.146995
ACTCTCC334850.013.14039411
TAGCGTA7550.013.11148237
ATAGGGG223700.013.04394738
TCACCCG140250.013.0223933
GGGAGGT383350.012.90230753
ATAGCTC127300.012.83012439
CCGGACA138450.012.46897912
TCACTCT361600.012.3916479
GTTGTCG19600.012.28761826
GGAGGTC366300.012.2643494
Produced by FastQC (version 0.11.5)