FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49353828
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT912180.18482456923098245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT164600.026.2282745
AGAACGA205300.021.0941544
AGACCGC225900.019.247884
CAGACCG228550.019.2117023
CCGCGTT234650.018.5288227
AAGAACG235750.018.3873343
CGCGTTC238900.018.208618
ACCGCGT242550.017.9536026
GACCGCG242550.017.9265985
AATACGG245650.017.56404932
ATACGGA245850.017.55921433
TACGGAG245800.017.52656734
CCGTACG83400.016.9109824
TTCGGCG81500.016.90079944
CGTACGC85150.016.6426775
CAATACG263850.016.51399431
TCAGACC273750.016.1717572
ACGGAGA279050.015.55956935
TCGGCGC90050.015.32139745
TCCGTAC94500.015.2578553
Produced by FastQC (version 0.11.5)