FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49353828
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG66750.09.99826938
GGCATAG74700.09.02679535
CATAGGG72450.08.96861937
TCAGACG78800.08.3022722
GCATAGG77700.08.24565336
CGTAACG21900.08.2265342
GCGTTCG16950.08.09752815
TAATCGG36600.07.92382434
ATAACGC32200.07.90648339
ATAGCTC58300.07.883560739
AGCTTAG85700.07.74446145
CAGACGT82900.07.56623553
TAACGCC32650.07.5215740
TAATTGA139100.07.4345884
ATAATCG43150.07.3983993
CGTAGGG31150.07.38777537
CTAGCAT84950.07.3592081
CGTTCGC18350.07.35711516
ACCCGGC69100.07.28638175
GTAACGA35850.07.28066318
Produced by FastQC (version 0.11.5)