FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48537573
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1120140.23077791714060364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC263650.022.01814
GACCGCG264850.021.7399335
CAGACCG271500.021.4400233
ACCGCGT271100.021.2793376
GAACGAT275800.020.97999445
CCGCGTT280850.020.514187
CGCGTTC282850.020.3929868
TCAGACC321550.018.6906342
GCGTTCT317300.018.3064949
AGAACGA322050.017.98065844
CTCAGAC373750.017.4177231
TACGGAG344200.016.41848834
AATACGG348150.016.33692732
ATACGGA349050.016.31331833
AAGAACG359800.016.06221443
CGTTCTC373250.016.03248410
CCGTACG57300.015.98187354
CGTACGC59550.015.5669435
CAATACG371300.015.54884531
CGGAGAG372850.015.10332536
Produced by FastQC (version 0.11.5)