FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48537573
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG498170.10263595173990261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG27000.016.33138726
GGGAGGT287000.013.8651843
ATAGGGG200800.013.10971438
AGCTTAG202900.012.875874545
GGAGGTC304250.012.7836234
GGCATAG203800.012.61873335
GTCACTC315050.011.9546958
TGTCGTT39150.011.43511128
GGTCACT337100.011.4192177
GAGCTTA228400.011.40876444
TGGCATA229250.011.35561834
TCACTCT339150.011.3514929
TGGGAGG261200.010.8458462
ACTCTCC363950.010.40483911
ATATAGC47100.010.3173486
GAGGTCA421250.010.0297275
CATAGGG261400.09.95013837
GCTCTGT397600.09.7001222
TCGTTCC45300.09.58505830
TCTCCCC398150.09.56154913
Produced by FastQC (version 0.11.5)