FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49689994
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1750400.3522640795649925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT321850.026.59299345
AGACCGC341100.025.184084
CAGACCG349400.024.6824743
GACCGCG362400.023.6417355
AGAACGA374700.022.84861844
ACCGCGT377850.022.7167246
CCGCGTT387700.022.1397
CGCGTTC399200.021.5126088
TCAGACC413950.021.0673962
AAGAACG410950.020.84421243
TACGGAG416250.020.51330234
AATACGG420000.020.42702732
ATACGGA421450.020.34058833
CTCAGAC460300.020.3280141
CAATACG430000.020.01493331
GCGTTCT433600.019.8527669
CCGTACG68900.019.005344
ACGGAGA452550.018.93265235
CGGAGAG458200.018.64617536
ACTCCCC473900.018.27447325
Produced by FastQC (version 0.11.5)