FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53600698
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG135250.015.6539145
ATAGGGG151600.014.143764538
GGCATAG148200.014.13425235
GAGCTTA163550.012.94519944
CATAGGG167550.012.77048537
TGGCATA173300.012.24291634
GCATAGG179800.011.86287636
GGGAGGT269650.011.7398823
GTCAGGA213400.010.709861
ATAGCTC70700.010.69279439
GGAGGTC287100.010.4542754
ACCCGGC77150.010.3238165
TCAGACG87850.010.2188222
GTCACTC304200.09.7932148
GTTGTCG19950.09.69897826
TAGCTCT78850.09.64464740
TCGCGCT42700.09.6425945
ACTCTCC312950.09.41154911
TAGGGGA304200.09.30449439
TCACCCG90550.08.9699263
Produced by FastQC (version 0.11.5)