FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49270442
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG499090.10129602653047033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG161550.014.97657945
ATAGGGG170550.014.31698138
GGCATAG176700.013.76874935
GGGAGGT274050.013.5665933
GAGCTTA191200.012.91258644
GTCACTC272250.012.8971478
GGTCACT273850.012.6987447
ACTCTCC282300.012.39821411
GTTGTCG27200.012.22349426
CATAGGG200200.012.15126937
TGGCATA207150.011.80988134
GGAGGTC311700.011.7044714
GCATAGG214900.011.42579236
TCACTCT318650.011.32228859
CACTCTC321150.010.98932110
GAGGTCA340850.010.7562135
TAGGGGA334850.010.42987839
CGTAGGG101350.010.14297637
AGCATAG103050.010.09338645
GCGTAGG102450.09.99897436
Produced by FastQC (version 0.11.5)