FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46180418
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1866960.40427524930588543No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA468010.10134382066442102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT328100.027.0095745
AGACCGC384750.023.1144584
CAGACCG388650.022.9230333
AGAACGA389550.022.78934144
ACCGCGT398100.022.2997726
GACCGCG399050.022.2354095
CCGCGTT407900.021.7419477
CGCGTTC410600.021.6263758
GCGTTCT437650.020.330839
AAGAACG437800.020.2725243
TCAGACC447600.020.24582
AATACGG448950.019.76179732
CTCAGAC482950.019.7185041
TACGGAG453100.019.58421334
ATACGGA457700.019.44145833
CAATACG465450.019.11597831
ACGGAGA476150.018.6030835
CCCAATA483150.018.53345329
CGGAGAG480500.018.36845836
ACTCCCC499350.018.10522725
Produced by FastQC (version 0.11.5)