FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46180418
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG24250.014.08085926
AGCTTAG119000.013.1767345
ATAGGGG126100.012.79596738
GGCATAG125850.012.65582235
GAGCTTA141200.011.31186344
TAGCGTA6950.011.10229437
CATAGGG146300.011.09124637
GGGAGGT227950.011.0080513
TGGCATA151100.010.67473934
GTCACTC240750.09.9842358
GCATAGG160650.09.98423536
GGTCACT239900.09.9540517
ATATAGC40650.09.8368566
AGGGATC124200.09.6948262
GGAGGTC259650.09.6468024
ACTCTCC252850.09.47981211
TCAGACG78250.09.1580532
GCGTTCG22250.09.08679615
TGTCGTT39850.08.9068928
ACCCGGC64050.08.8034215
Produced by FastQC (version 0.11.5)