FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43712291
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG139900.015.13400745
GGCATAG152800.013.78275535
ATAGGGG161100.013.14248838
GAGCTTA169750.012.39321144
TGGCATA173950.012.18456534
GGGAGGT267800.012.09013753
GGTCACT244300.011.93608957
GTTGTCG20800.011.89902626
GGAGGTC259100.011.6971814
CATAGGG184500.011.58539337
ACCCGGC72300.011.5456165
ACTCTCC260800.011.47429811
GCATAGG192000.011.15627736
GTCACTC262700.011.1257578
ATAGCTC70950.010.56025439
TCACTCT292950.010.3302269
GAGGTCA301800.010.2733365
CACTCTC292050.010.05391810
TCACCCG88250.010.0452923
TAGCGTA6500.010.03848537
Produced by FastQC (version 0.11.5)