FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43836941
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1815930.41424651414431496No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT609700.1390836098714096No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT559920.12772789050221364No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTG555910.12681313689292326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT357150.025.735861
CCGGCTT410100.024.0175742
TTCACGC433200.022.6454037
TCACGCT444750.022.0016758
TTCGACG24050.021.699786
CGGCTTC467550.021.4419233
ACGCTCA496800.019.88219310
CACGCTC502350.019.5908859
AACGCTA489450.019.56546623
ACGCTAC491600.019.53601624
CGGTATA36650.019.45956420
CGCTACC497050.019.29481725
AGACCGC171000.018.9926914
AAAACGC508400.018.90537621
GAAAACG506300.018.87218520
AAACGCT515700.018.60812822
CAGACCG177800.018.3306713
GGCTTCA561150.018.1611674
CGTTACG13800.017.77496321
TCGGCGC107250.017.74391245
Produced by FastQC (version 0.11.5)