FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43836941
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG555480.12671504610688963No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA538900.12293284789191836No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG528400.12053760776784127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT234700.014.4377413
GGAGGTC265650.012.71344
TCAGACG108450.012.0355852
AGGGATC165400.011.864512
TGGGAGG236750.011.3496682
ACTCTCC303250.010.850450511
CAGGGAT198250.010.7630531
GTCACTC315600.010.6172758
TGCCCGT172300.010.41512244
GGTCACT316500.010.3950857
CGTGGCG187800.010.3432141
ATAGGGG249150.010.24215138
CAGACGT130050.010.0728641
AGCTTAG248600.010.02663445
GGCATAG248850.010.01002335
GTCAGGA126900.09.8972121
TGGCATA263000.09.59080734
CTGGGAG315400.09.4771261
CACTCTC353800.09.4617610
GTAGCGT235550.09.32986645
Produced by FastQC (version 0.11.5)