FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44839038
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT449550.10025861839408777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC109900.023.2775544
CAGACCG117350.021.8956363
GACCGCG118050.021.803925
ACCGCGT120900.021.3271526
CCGCGTT129400.020.0305447
CGCGTTC120250.019.1971538
GAACGAT154300.016.75439345
TCAGACC162100.016.2674372
CCCGTAT42350.016.1641641
GCGTTCT153350.015.1708989
AATACGG169550.015.15454532
CGTTACG27000.015.08305621
CCGTACG46600.014.9192544
TACGGAG171700.014.91236434
CTCAGAC208500.014.860911
ATACGGA173550.014.83118833
ACGCGTC8400.014.731873540
AGAACGA177400.014.63614644
GTTCCTA68300.014.32989815
CCCTAAC27350.013.9966681
Produced by FastQC (version 0.11.5)