FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44839038
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT239550.013.0720133
GGCATAG156250.012.94541335
ATAGGGG160350.012.83860138
GGAGGTC253700.012.1564494
GGTCACT237650.012.1269797
AGCTTAG174000.011.98650645
GTCACTC245950.011.891248
GAGCTTA176150.011.58439244
TGGCATA177950.011.40471234
GAGGTCA300950.010.5167045
TCACTCT284800.010.4903319
ACTCTCC283500.010.4354611
AGGTCAC289550.010.4275186
CATAGGG197750.010.38775737
GCATAGG206150.09.99767536
AGGGATC108350.09.8898452
CGTAACG18700.09.74559842
TAGGGGA303900.09.58748339
GCTCTGT315500.09.35563722
TGGGAGG235400.09.3337222
Produced by FastQC (version 0.11.5)