FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47245840
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1007970.2133457675850403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC222650.021.0171974
GAACGAT222300.020.71583445
CAGACCG227200.020.6681523
GACCGCG228150.020.4708125
ACCGCGT240250.019.4303076
CCGCGTT250850.018.6092557
CGCGTTC253650.018.394968
CCGTACG37250.017.8168094
CGTACGC38150.017.6321335
AGAACGA268150.017.1569344
TCAGACC286450.016.8171022
GCGTTCT279450.016.7133459
CTCAGAC324500.016.339081
AATACGG289900.015.94500932
TACGGAG292250.015.82394234
AAGAACG292300.015.73196843
ATACGGA295400.015.70097733
CTCCGAT49750.015.1601381
CAATACG304600.015.15369131
ACGGAGA308200.014.9755935
Produced by FastQC (version 0.11.5)