FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47245840
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG119350.013.15769738
GGCATAG133650.011.61502435
GGGAGGT192700.011.0618943
AGCTTAG140150.011.01286745
GAGCTTA148350.010.57074144
GTTGTCG21050.010.36802226
CATAGGG154500.010.20795337
GTCACTC202900.09.9458758
GGAGGTC212000.09.9448434
TGGCATA162550.09.75756934
GCATAGG162200.09.69519736
ACTCTCC218450.09.57743611
TAATCGG37150.09.0850364
GGTCACT229150.09.0223087
CACTCTC238200.08.53736610
GAGGTCA264450.08.4825875
TCACTCT249400.08.3885249
AGGGATC129000.08.3085422
AATCGGT47650.08.216055
AGGTCAC274200.08.0901546