FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49058467
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG124800.012.58293745
ATAGGGG126650.012.43547638
GGCATAG129950.011.99872135
ACCCGGC72250.011.1244245
GAGCTTA143750.011.11209944
CATAGGG143200.011.07693837
GTTGTCG20100.010.744246526
GGGAGGT225900.010.64495853
TGGCATA151500.010.42542234
GCATAGG153300.010.33272236
GGAGGTC238850.09.6434654
ATAGCTC75300.09.62125739
TCACCCG93650.09.3525673
CGTAACG13850.09.09659842
ACTCTCC246850.09.07948211
ACACGGA90450.08.880650516
ACGGACA90250.08.87534518
TCAGACG73550.08.8166972
TAGCTCT84700.08.7660840
CACTCTC260750.08.672976510
Produced by FastQC (version 0.11.5)