FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47232667
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCT726840.15388502199124177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGGA246200.015.9544641
ACCCGGC95000.015.5131135
TCACCCG108150.014.4323943
ATAGCTC98400.014.08384739
CCGGACA112500.013.3834112
CACCCGG114300.013.3658634
AGCTTAG87650.013.1511245
ACGGACA113750.012.71670318
TAGCTCT111350.012.52533340
GGCATAG92100.012.4839335
CGGACAC121250.012.36174113
CGGACAG117850.012.236279519
ACACGGA119400.012.15283916
TGATAGC121250.012.11773537
CACGGAC123200.011.96059917
TTGATAG123050.011.90390436
CATAGGG98650.011.60942637
GCATAGG103550.010.97314936
GCCCGGA142600.010.82700510
TAGCGTA7750.010.74212137
Produced by FastQC (version 0.11.5)