FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45946956
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT609130.13257243853107484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC124050.023.0221844
CAGACCG126900.022.5760523
GAACGAT133100.021.39993945
ACCGCGT137450.020.8705866
CCGCGTT140400.020.448097
CGCGTTC141200.020.347758
GACCGCG141850.020.1809625
AGAACGA154850.018.39413644
TTCGGCG68000.017.99893844
AATACGG159400.017.92591332
ATACGGA162650.017.56772433
CCGTACG71050.017.2629134
CGTACGC71850.017.1647645
TCAGACC175000.016.7438032
TACGGAG170900.016.60117734
CAATACG175600.016.3747131
TCGGCGC75000.016.2890445
GCGTTCT182650.015.8163249
AACGCTA90350.015.7883923
AAGAACG182650.015.64375343
Produced by FastQC (version 0.11.5)