FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45946956
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG127050.014.69860545
GGCATAG131150.014.0812135
ATAGGGG137350.013.62721638
TGGCATA150500.012.31563534
GAGCTTA153100.012.16820144
GGGAGGT247450.012.0511383
CATAGGG159850.011.65291137
GTCACTC244400.011.5567738
GCATAGG168650.011.04425936
ACTCTCC258000.011.00117111
GGAGGTC264050.010.9349774
GGTCACT255450.010.8506847
ACCCGGC70600.010.7424315
CACTCTC284900.09.97004110
TAGGGGA278400.09.81809139
ATAGCTC70250.09.54311239
TCACTCT311150.09.5259739
CGTAGGG93900.09.39154937
GTTGTCG16450.09.30023426
GCTCTGT303800.09.21622222
Produced by FastQC (version 0.11.5)