FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47579020
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1055040.22174479423914153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT221900.022.73246845
AGACCGC278300.018.9568024
CAGACCG285900.018.4843543
AGAACGA273200.018.4683444
GACCGCG311800.016.897915
AAGAACG311600.016.18517743
ACCGCGT327650.016.1074186
CCGTACG53200.015.8159554
CCGCGTT341050.015.5196947
GCGTTCT342300.015.4762619
CCCGTAT33150.015.4757781
CGTACGC54950.015.4755585
CGCGTTC342350.015.4411498
CTCAGAC370700.015.417441
TCAGACC356250.015.1309922
ATACGGA352850.014.91489933
TACGGAG354000.014.85710634
AATACGG355900.014.79972332
AGACGCT164950.014.53731441
CCCGGAT133050.014.4425581
Produced by FastQC (version 0.11.5)