FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47420742
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG479750.10116880921011316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG168850.014.48961745
ATAGGGG173850.014.11273238
GGCATAG175450.013.77524935
GGGAGGT272200.012.9128923
GAGCTTA194600.012.52478644
GTTGTCG21050.012.50478226
GGTCACT260150.012.4794887
GTCACTC262400.012.3635558
TGGCATA200150.012.26649534
CATAGGG208400.011.75146237
GGAGGTC286350.011.7245274
ACTCTCC284850.011.56503511
GCATAGG217650.011.22892236
GAGGTCA310700.010.9928465
TCACTCT308950.010.7683539
CACTCTC305750.010.65861910
ACCCGGC69950.010.6146545
AGGTCAC328400.010.3796386
TAGGGGA324950.09.9609739
TGGGAGG272400.09.7644832
Produced by FastQC (version 0.11.5)