FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46302807
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT723320.1562151512758179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT131950.025.34960745
AGAACGA171500.019.58330544
AGACCGC178050.018.6486724
CAGACCG184850.018.0236363
TTCGACG16050.016.8188256
GACCGCG203050.016.385755
ATACGGA204950.016.31122633
CCGCGTT205050.016.247367
TACGGAG205950.016.16552534
AATACGG206300.016.15106232
AAGAACG208950.016.1272343
ACCGCGT207000.016.116116
CGCGTTC215150.015.4951348
CCGTACG82350.015.2977624
CGTACGC82850.015.1510525
GTTCCTA70500.015.124351515
CAATACG223750.015.01364331
TCAGACC232200.014.6582962
ACGTTCG13350.014.4912766
ACGGAGA233350.014.36372135
Produced by FastQC (version 0.11.5)