FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46302807
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG101100.012.05510345
ATAGGGG102850.011.94646538
GGCATAG103150.011.67171435
CATAGGG110300.011.01712737
ACCCGGC70800.010.4571575
GTTGTCG19800.010.45390526
GCATAGG119600.010.12281236
GAGCTTA124100.09.91117144
ATAGCTC69900.09.49730939
TAATCGG33100.09.45033554
ACTCTCC178150.09.41725811
TGGCATA129000.09.34930934
GTCACTC181450.09.30213458
GGTCACT178750.09.279087
GGGAGGT201100.09.2780373
TCAGACG85050.09.1038292
TAGCTCT81400.08.70930140
ACACTCG87250.08.6921044
CACTCGA81250.08.5582735
CACCCGG96450.08.399644
Produced by FastQC (version 0.11.5)