FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53347812
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1037050.1943941018611972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC231500.024.4045334
CAGACCG241050.023.4843393
ACCGCGT250750.022.6386816
GACCGCG250650.022.6207835
GAACGAT230350.022.27006345
CCGCGTT257150.022.1714927
CGCGTTC266150.021.4302028
GCGTTCT296500.019.3428279
AGAACGA266800.019.31187644
TCAGACC301500.019.2086162
TACGGAG278250.018.48484834
AATACGG279850.018.45152332
ATACGGA281100.018.3694733
CTCAGAC363800.017.349341
ACGGAGA301700.017.13012335
CAATACG307750.016.87378531
TTCGGCG96000.016.66376744
AAGAACG316100.016.35687643
CGGAGAG313900.016.3281636
CCCAATA328200.015.91836429
Produced by FastQC (version 0.11.5)