FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53347812
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC91600.013.369465
TCACCCG106750.012.3793363
AGCTTAG113350.011.79030345
ATAGGGG124750.011.01949138
CACCCGG117250.011.0018384
GGCATAG120050.010.96402735
ATAGCTC106800.010.63851539
GTTGTCG21300.010.45651126
TAGCTCT112850.010.14792240
TAGCGTA8050.010.06177137
ACACGGA119600.09.95111916
CATAGGG135200.09.93494537
ACGGACA127050.09.93424418
CACGGAC119100.09.917335517
CGGACAG119850.09.855144519
TGATAGC123900.09.6606937
TCAGACG85650.09.6201292
GAGCTTA141550.09.60001944
CCGGACA133150.09.59717912
GGGAGGT238700.09.5728393
Produced by FastQC (version 0.11.5)