FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46204546
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGCG71750.016.96463444
GTCCGTT38600.016.7409521
CCCGTAT34700.014.8590681
AGACCGC83450.014.6681244
CGGATAC67000.014.4073653
TACGGTA67500.014.26622844
AACGCTA96400.014.237082523
CCCGGAT140650.014.0392991
CCGTACG88200.013.8781764
ACGCTAC99350.013.85963424
CCTTACG70050.013.74690141
TTACGGT71800.013.53718943
CGTTACG10000.013.49962921
CGTACGC92450.013.3862165
CAGACCG93050.013.324083
GTTCCTA63400.013.30800715
GTCCGAT28800.013.212341
CGCTACC104700.013.08707525
GCGGACT66200.013.085952
ACGTTCG12350.012.9347966
Produced by FastQC (version 0.11.5)