FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46572672
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA563590.12101302669514002No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG504590.10834465327649656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG26000.017.39253226
ATAGGGG158950.015.38354638
AGCTTAG163450.014.85198645
GGCATAG163400.014.75821835
GGGAGGT266900.013.6863843
GAGCTTA182800.013.328382544
GTTCTAT225650.013.1010861
TGGCATA185700.012.99754234
CATAGGG191750.012.75216437
GGAGGTC288450.012.4176854
GTCACTC281250.012.2735318
TGTCGTT36350.012.06745728
AGGGATC162750.011.9897812
GCATAGG209300.011.66115136
GGTCACT304400.011.4735117
ACTCTCC306500.011.277863511
ATATAGC43550.011.15996
TCGTTCC41700.010.84234330
CAGGGAT206100.010.62949851
TCTCCCC334700.010.52274113
Produced by FastQC (version 0.11.5)