FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47529164
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA538270.11325046659772933No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG493280.1037846994321213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT261850.012.4879633
GTTGTCG23200.012.22637426
AGCTTAG178200.012.118402545
ATAGGGG182150.011.99288438
GGCATAG184650.011.5998635
GGAGGTC284300.011.550634
GGTCACT295550.010.6977837
TGGCATA205250.010.63281334
GAGCTTA205050.010.50941244
ACTCTCC306250.010.30301511
GTCACTC307250.010.2983798
AGGGATC190250.09.8340662
CACTCTC328750.09.60471210
CATAGGG229250.09.58851337
TATCCTA96900.09.1919765
GCATAGG238800.09.15794436
TGGGAGG265400.08.9813052
TAGGCTA90350.08.96740410
ATCCTAG108450.08.9203916
TCACTCT364600.08.9131379
Produced by FastQC (version 0.11.5)