FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42278967
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1086850.2570663564225682No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG628790.14872406887330053No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG534890.12651444393142341No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA478790.11324543478084505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG28600.024.3043546
GAACGAT225150.023.44064345
TCGACGA37800.018.9245647
CGACGAG38050.018.3864088
TCACGCT224000.017.8455758
CGTTACG21550.017.64695521
GCCGGCT201450.017.4264641
AGAACGA305050.017.38121644
CCGGCTT253750.016.5428812
CGGTATA55700.016.4006920
ACGCTCA255200.016.0957510
AGACCGC330050.015.9969374
CGGCTTC269450.015.9713753
GTTCCTA86600.015.923305515
CAGACCG333500.015.9192163
AACGCTA248200.015.616423
GACCGCG338500.015.5709285
ACGCTAC249950.015.54354524
TTCACGC260500.015.5264657
ACCGCGT344400.015.36263756
Produced by FastQC (version 0.11.5)