FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42278967
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG508350.1202370909393316No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA498440.11789313584695672No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG474570.11224730254171063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG28750.015.57253926
AGCTTAG188950.012.85223845
GCGTCGT25450.012.54903411
GGGAGGT280350.012.5069923
ATATAGC44250.012.2049796
GGTCACT270550.012.1525257
ACCCGGC70400.012.1458345
GGCATAG198500.012.03937935
GAGGTCA282600.011.9834185
GGAGGTC284150.011.9421064
CGCCGTA12100.011.91520545
TGTCGTT37400.011.91099728
TCAGACG101450.011.8025122
GAGCTTA206300.011.66165944
ACTCTCC291100.011.35755511
ATAGGGG217900.011.24631538
GCTAAGT43950.011.20715814
GTCGTTC40450.011.01288829
GTCACTC302800.010.9770438
TGGCATA218600.010.93132234
Produced by FastQC (version 0.11.5)