FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40599269
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2043810.5034105417021179No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1000680.2464773442103108No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA425640.10483932604796406No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT423580.10433192774973363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG417800.026.7108525
CAGACCG427600.026.4670013
ACCGCGT423600.026.3504316
AGACCGC429900.026.2835334
TCAGACC459200.025.4345252
CCGCGTT441450.025.4123747
CGCGTTC449650.024.9689588
CTCAGAC512450.023.786161
GCGTTCT491050.022.9600689
GAACGAT525100.021.44551845
CGTTCTC545650.021.4419210
GCCGGCT338450.021.0979161
CCGGCTT412100.019.7478752
TTCACGC414800.019.4891537
CCTCTCA618350.019.1138219
AGAACGA593850.019.04612444
TCACGCT426900.019.0368928
GTTCCTA117150.018.99452615
AATACGG596450.018.66886332
ATACGGA598150.018.54809833
Produced by FastQC (version 0.11.5)