FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40599269
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG725060.1785894223859055No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA641570.15802501271636196No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG597490.1471676743736445No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT462970.11403407287948952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCAC344150.016.180166
GGGAGGT360800.016.073113
GTTGTCG29650.015.706449526
GGTCACT348500.015.687267
GGAGGTC363050.015.6385334
GTCACTC352600.015.2874588
GAGGTCA379600.014.9739175
AGCTTAG273300.014.83458345
GGCATAG272850.014.51322535
ATAGGGG289700.014.12689138
TGGCATA291200.013.69139134
TCACTCT402550.013.5636799
GAGCTTA297400.013.45810844
TGGGAGG311750.013.373812
ACTCTCC404500.013.37066911
TTGTCGT37600.013.22320827
CACTCTC411200.012.922811510
ATATAGC42600.012.6224516
CTGGGAG365100.012.4860191
AGGGATC172550.012.4206012
Produced by FastQC (version 0.11.5)