FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49373370
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG110600.012.93512945
ATAGGGG120150.012.32319738
GGCATAG120900.011.91149135
CATAGGG134000.010.96557637
GCGTTCG31200.010.81803115
GAGCTTA134200.010.69405244
GTCACTC216650.010.4157888
GGGAGGT242850.010.38849453
GCATAGG144000.010.2353336
ACCCGGC76550.010.1066385
CCGCGTT35150.010.05134713
GTTGTCG20050.09.87968326
CGCGTTC34500.09.84845514
CGTTCGC35450.09.83845916
TGGCATA150650.09.75332134
ACTCTCC238900.09.62622511
CGTAGGG77700.09.55687537
GCGTAGG79750.09.36764536
TAGGGGA236950.09.34309939
GGCGTAG81000.09.27841835
Produced by FastQC (version 0.11.5)