FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49145043
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG521930.10620196222027928No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG513270.10443983129692246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG181100.013.88187945
ATAGGGG194550.013.07832438
GGCATAG191950.013.07815435
GGGAGGT300300.012.7958613
TGGCATA208300.012.0623934
GGAGGTC313800.011.9798374
GAGCTTA212450.011.93934944
TATCCTA105150.011.5166765
TAGGCTA106500.010.94627310
GTTGTCG21500.010.67137926
CATAGGG239000.010.6271537
ATCCTAG123650.010.5029986
CCTAGGC111000.010.4822298
AGGGATC137250.010.4148432
GTATCCT117050.010.3658454
TAGCGTA6750.010.33186437
TGTATCC119800.010.3158943
ACTCTCC366100.010.19826111
GCATAGG254100.09.99451736
CTAGGCT116450.09.933679
Produced by FastQC (version 0.11.5)