FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50932415
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC108300.019.6726234
TTCGACG27650.018.4701946
AACGCTA136500.018.2946223
CAGACCG117300.018.2589513
ACGCTAC138250.018.12923824
CGCTACC144250.017.25162325
GCCGGCT140700.016.9686991
CCGTACG30800.016.8003334
CCGGCTT167800.016.638632
CCCGTAT30450.016.4056321
TTCACGC161550.016.3900227
CCCGGAT155250.016.37851
TCACGCT159850.016.2547158
CGGATAC97100.016.172353
CGGTATA41050.016.1119320
AAAACGC158450.015.8731321
CTCCGAT48450.015.6053231
GAAAACG158800.015.52649820
CCGGATA109750.015.4972622
TTCGCCA97300.015.4443114
Produced by FastQC (version 0.11.5)