FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50932415
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG27750.013.29837526
AGCTTAG156950.013.10470845
ATAGGGG161700.013.05621538
GGGAGGT219550.012.9264923
GGCATAG165150.012.59106235
GAGCTTA178050.011.75427544
GGTCACT236900.011.3939037
GTCACTC246100.011.0421688
GGAGGTC266100.010.9285944
ACTCTCC253950.010.82628911
GAGGTCA267000.010.782465
CATAGGG196550.010.63821837
TGGCATA197500.010.539927534
AGGTCAC271750.010.4865816
GCATAGG204250.010.25792636
TCACTCT273450.010.1358459
CACTCTC280500.09.62491610
TGTCGTT40350.09.42454628
TGGGAGG245450.09.326682
ATATAGC50600.09.2456986
Produced by FastQC (version 0.11.5)