FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46603439
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1248740.2679501828180534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG50300.027.5080766
GACCGCG248950.024.9882645
ACCGCGT249300.024.9261076
AGACCGC251100.024.8643054
TCGACGA56500.024.6885917
CAGACCG262000.023.9329243
CCGCGTT262650.023.684867
CGCGTTC264800.023.5176378
GTTCGAC60550.022.665685
CGACGAG60450.022.5539158
CGGTATA75850.022.27880920
GAACGAT275900.022.19637545
TCAGACC292450.021.7841262
GCGTTCT315100.019.8705759
CTCAGAC367050.018.9210431
AGTTCGA75100.018.7240644
AATACGG334300.018.48148232
AGAACGA335850.018.30795744
TACGGAG339500.018.13116334
ATACGGA341550.018.07519133
Produced by FastQC (version 0.11.5)